Analysis of coverage.

As each clone is not completely sequenced, there is a greater coverage of clones than sequences in the assembly. We assume a 500-bp average read length and 3.5-Gbp genome size.

Vector typeInsert size (kbp)Number ofCoverage (×)
ClonesSequencesSequencesClones
High-copy plasmid230,000,00060,000,0008.517
Low-copy plasmid105,000,00010,000,0001.414
BAC150300,000600,0000.115
Total 35,300,000 70,600,000 10 46