Table 2

Expression of sGFP from compensatory mutations between the predicted loop sequence of the RCNMV RNA-2 trans-activator and the complementary RCNMV RNA-1 8-nt element in the CP subgenomic promoter. Mutated nucleotides are in bold typeface and underlined.

RCNMV RNA-1/TBSV inoculumRNA-1 8-nt element RNA-2 TA loop sequencesGFP produced*
R1sGFP/pHST2-SL25′-GGGGCGAT-3′5′-ATCGCCCC-3′+++
R1sGFP-M/pHST2-SL2CGGCCGTT ATCGCCCC
R1sGFP/pHST2-SL2-M GGGGCGAT AACGGCCG
R1sGFP-M/pHST2-SL2-MCGGCCGTT AACGGCCG +++
  • * sGFP production relative to induction by RNA-2: +++, 100%; –, not detected.