Table 1

Inhibition of m1–7 peptide binding to DRB1*0401 (15) by 20-residue peptides of OspA.

OspA peptide*IC50 (μM)Nine-residue peptides with appropriate p1 anchor residueDR4-algorithm scores for peptides with an appropriate p1 anchor residue
154–1734.381161, 162, 165, 166(−)  0.4,  (−)  0.8,  (+)  6.5,  (−)  5.4
 54–73>10054, 55, 58, 61, 63(−)  1.1,  (−)  4.1,  (−)  6.3,  (−)  2.8,  (−)  0.1
 74–93>10075, 76, 79, 86(−)  6.6,  (+)  1.1,  (+)  2.4,  (−)  1.9
124–143>100126, 132, 136, 137(−)  4.7,  (−)  4.3,  (−)  3.3,  (−)  2.4
  • * Testing was limited to peptides with both sufficient quantity of material and a broad range of DR4-predicted binding scores. OspA164–183 was not available for testing.

  • The number of potential DRB1*0401-binding 9-residue peptides contained within a 20-residue sequence was determined by the presence of an appropriate p1 anchor residue (F, I, L, M, V, T, or Y). p1 anchor residue amino acid numbers are listed for each candidate peptide.

  • Scores were calculated for OspA nine-residue peptides beginning with F, I, L, M, V, T, or Y (9). Scores are listed, respectively, for each 9-mer peptide contained within the 20-mer peptide tested.