Table 1

R-CmPP16-1 interacts with mesophyll plasmodesmata to mediate its own cell-to-cell transport and potentiate the trafficking of sense and antisense RNA. Lucifer yellow CH and 11-kD FITC-dextran were from Molecular Probes. TOTO, TOTO-1 iodide; TRITC, tetramethylrhodamine-5-(and 6)-isothiocyanate.

Injected materialMicroinjection
Total (n)Movement*[n (%)]
Lucifer yellow CH 3231 (97)
FITC-dextran (11 kD) 40 1 (2.5)
R-CmPP16-1 + 11-kD FITC-dextran 25 21 (84)
Endogenous CmPP16 + 11-kD FITC-dextran 60 48 (80)
TRITC–R-CmPP16-1 2117 (80)
TRITC–R-CmPP16-1 + sense CmPP16-1 RNA-CF 2015 (75)
TRITC–R-CmPP16-1 + antisense CmPP16-1 RNA-CF 2015 (75)
TRITC–R-CmPP16–1 + RCNMV sense RNA 2-CF 10 8 (80)
CmPP16-1 ss-DNA-TOTO 10 0 (0)
TRITC–R-CmPP16-1 + CmPP16-1 ss-DNA-TOTO 10 0 (0)
TRITC–RCNMV MP 10 8 (80)
TRITC–RCNMV MP + CmPP16-1 sense RNA-CF 10 8 (80)
CmPP16-1 sense RNA-CF 20 3 (15)
CmPP16-1 antisense RNA-CF 20 2 (10)
RCNMV sense RNA 2-CF 10 1 (10)
SUT1 sense RNA-CF 10 1 (10)
SUT1 RNA-CF + TRITC–R-CmPP16-1 10 10 (100)
  • * Number of injections and percent of total injections in which the probe moved into surrounding mesophyll cells. In coinjection experiments, the distribution patterns of TRITC-labeled protein and CF-labeled RNA were coincident, as deduced from simultaneous multiple-channel fluorescence analysis performed with a CLSM [see supplementary material (Fig. 3) atwww.sciencemag.org/feature/data/982968.shl].

  • SUT1 from potato (6) was CF labeled as described in (18).