Table 2

Distribution of 14C in acetate produced from 14CO2 by termite gut spirochetes. Acetate from the cultures shown in Table 1 (0.5 to 1.0 μmol each) was degraded as previously described (17). Recovery of the 14C label from methyl- and carboxyl-labeled acetate standards (American Radiolabelled Chemicals) was 92.4 and 98.5%, respectively, with <1% cross contamination.

Origin of acetate sampleSample radioactivity (dpm)14C-dpm recovered inTotal14C dpm recovered (%)
CH3 group (%)COOH group (%)
ZAS-2 culture
(H2,CO2–grown)16,4907,896  (47.9)7,938  (48.1)15,834  (96.0)
ZAS-1 culture
(N2,CO2–grown)3,4381,228  (35.7)2,102  (61.1)3,330  (96.8)
ZAS-1 culture
(H2,CO2–grown)9,5353,488  (36.6)5,286  (55.4)8,774  (92.0)