Table 1

G418-resistant cell clones obtained after transfection of Huh-7 cells with in vitro–transcribed HCV replicon RNAs. The number of viable cell clones after subpassage of clones obtained in experiment 1 is given in the right column.

Construct ExperimentSub- passages (exp. 1)
1 2 3
I377neo/NS2-3′/wt 12 2038 1
I377neo/NS2-3′/Δ 0 3 8 0
I377neo/NS3-3′/wt 20 >60 40 2
I377neo/NS3-3′/Δ 2 8 18 0
I389neo/NS2-3′/wt 6 20 25 1
I389neo/NS2-3′/Δ 1 10 4 1
I389neo/NS3-3′/wt 30 15 17 5
I389neo/NS3-3'/Δ 1 2 6 0