Table 1

Characteristics of experimental animals and BrdU-labeled cells in the principal sulcus region. All animals, except #7, were given one to five i.p. injections (injs.) daily of 75 to 100 mg/kg BrdU. For all animals but #7, survival time represents the time after the last BrdU injection when the monkey was perfused. Animal #7 received four i.v. injections of 100 mg/kg BrdU, each separated by 1 week with the last injection 24 hours before perfusion. Animals #1 to #6 were also used in a different study of neurogenesis in the dentate gyrus (7). The stereological optical dissector method was used to estimate the number of BrdU-labeled cells/mm3(19). The percentages of BrdU-labeled cells that expressed specific markers were each obtained from a sample of 100 BrdU-labeled cells per marker per animal. As mentioned in the text and in (22), three additional male animals (#10, #11, #12) were used for a retrograde tracer study and for identifying new neurons.

No. (sex)Age (years)BrdU injs.Survival timePercent BrdU-labeled cellsNumber of BrdU-labeled cells/mm3
NeuN NSE MAP-2 GFAP
1 (m)5 1 2 hours 0 0 0 200.4
2 (m) 16 1 2 hours 0 0 0 310.6
3 (m) 5 5 2 weeks 52 43 37 526.5
4 (f) 7 5 2 weeks 47 39 33 813.2
5 (m) 10 3 1 week 38 32 26 417.6
6 (f) 15 5 2 weeks28213.7
7 (m) 15 4 24 hours 53 48 29 315.6
8 (m) 5 1 1 week7.2
9 (m) 5 1 2 weeks14.4