Table 1

Altered numbers of hematopoietic cells inbim / mice. The cellular composition of blood, spleen, and thymus for young adultbim −/− mice and their WT littermates was determined by cell counting and immunofluorescence staining with monoclonal antibodies specific for leukocyte subsets, followed by flow cytometry (23). All differences shown are statistically significant (P < 0.05).

Cell typeNo. of cells per milliliter × 10−6
bim+/+bim−/−
Blood
Red blood cells7600   ± 700 8400   ± 1000  
Total leukocytes 6.3 ±  0.9  22   ±    7  
B cells 3.1 ±  0.5  10.7 ±    4  
CD4+8 1.0 ±  0.4   2.2 ±    0.7
CD48+ 0.6 ±  0.2   2.2 ±  0.7
Monocytes 0.7 ±  0.2   3.0 ±  1.2
Granulocytes 0.7 ±  0.3   1.7 ±  0.5
Platelets 880   ± 100   460   ±   60  
Spleen
Total cells 160   ±  40   380   ±  110  
B cells  97   ±  10   220   ±  100  
CD4+8   26   ±   2    69   ±   10  
CD48+   13   ±   2    32   ±    5  
Granulocytes  10   ±   1    32   ±    5  
Thymus
Total cells 200   ±  40   120   ±   50  
CD48    5   ±   2    12   ±    8  
CD4+8+  170   ±  33    70   ±   23  
CD4+8   13   ±   4    30   ±   11  
CD48+    4   ±   1    12   ±    7