Table 2

Cell populations in PI3Kγ-deficient mice. Seven- to 12-week-old PI3Kγ +/− andPI3Kγ −/− littermate mice were used. Total cells from thymi (n = 7), spleens (n = 7), lymph nodes (n = 3), and bone marrows (n = 3) were stained with antibodies against the indicated proteins. Populations were determined by FACScan. Bold numbers indicate statistically significant differences betweenPI3Kγ +/− andPI3Kγ −/− mice (Mann-Whitney Utest; P < 0.05). Values are given as the mean ± SEM.

SubsetsPI3Kγ+/− (% of total)PI3Kγ−/− (% of total)
Thymus
CD4+CD8+ 82.3 ± 1.1077.8 ± 3.25
CD4+CD8 10.2 ± 0.849.77 ± 1.18
CD4CD8+ 1.90 ± 0.082.94 ± 0.31
CD4CD8 5.55 ± 0.259.48 ± 1.78
Spleen
CD4+CD8 23.9 ± 2.4512.9 ± 2.35
CD4CD8+ 8.55 ± 0.966.45 ± 1.03
B220+slgM+ 34.6 ± 3.1235.3 ± 1.24
Mac1+Gr1+ 1.68 ± 0.113.65 ± 0.79
Lymph node
CD4+CD8 59.9 ± 2.8752.3 ± 3.43
CD4CD8+ 15.4 ± 1.5313.8 ± 0.91
Bone marrow
B220+CD43+ 9.70 ± 1.0310.7 ± 0.35
B220+CD43 20.8 ± 5.1520.4 ± 5.47
Mac1+Gr1+ 45.6 ± 7.5145.3 ± 7.43