Cell populations in PI3Kγ-deficient mice. Seven- to 12-week-old PI3Kγ +/− andPI3Kγ −/− littermate mice were used. Total cells from thymi (n = 7), spleens (n = 7), lymph nodes (n = 3), and bone marrows (n = 3) were stained with antibodies against the indicated proteins. Populations were determined by FACScan. Bold numbers indicate statistically significant differences betweenPI3Kγ +/− andPI3Kγ −/− mice (Mann-Whitney Utest; P < 0.05). Values are given as the mean ± SEM.
| Subsets | PI3Kγ+/− (% of total) | PI3Kγ−/− (% of total) |
|---|---|---|
| Thymus | ||
| CD4+CD8+ | 82.3 ± 1.10 | 77.8 ± 3.25 |
| CD4+CD8− | 10.2 ± 0.84 | 9.77 ± 1.18 |
| CD4−CD8+ | 1.90 ± 0.08 | 2.94 ± 0.31 |
| CD4−CD8− | 5.55 ± 0.25 | 9.48 ± 1.78 |
| Spleen | ||
| CD4+CD8− | 23.9 ± 2.45 | 12.9 ± 2.35 |
| CD4−CD8+ | 8.55 ± 0.96 | 6.45 ± 1.03 |
| B220+slgM+ | 34.6 ± 3.12 | 35.3 ± 1.24 |
| Mac1+Gr1+ | 1.68 ± 0.11 | 3.65 ± 0.79 |
| Lymph node | ||
| CD4+CD8− | 59.9 ± 2.87 | 52.3 ± 3.43 |
| CD4−CD8+ | 15.4 ± 1.53 | 13.8 ± 0.91 |
| Bone marrow | ||
| B220+CD43+ | 9.70 ± 1.03 | 10.7 ± 0.35 |
| B220+CD43− | 20.8 ± 5.15 | 20.4 ± 5.47 |
| Mac1+Gr1+ | 45.6 ± 7.51 | 45.3 ± 7.43 |