Numbers of lymphoid cells inPI3Kγ −/− mice. Thymi (n = 12), spleens (n = 5), and all lymph nodes (n= 5) from 4- to 7-week-old PI3Kγ +/− andPI3Kγ −/− littermate mice or 6-week-oldPI3Kγ +/− chimeric andPI3Kγ −/− chimeric mice (n = 4 for all organs) were analyzed. Bold numbers indicate statistically significant differences between PI3Kγ +/− andPI3Kγ −/− mice or betweenPI3Kγ +/− → rag1 −/−and PI3Kγ −/− →rag1 −/− chimeric mice (Mann-WhitneyU test; P < 0.05). Values are given as the mean ± SEM.
| Lymphatic organ | PI3Kγ+/− | PI3Kγ−/− | PI3Kγ+/− → rag1−/− | PI3Kγ−/− → rag1−/− |
|---|---|---|---|---|
| Thymus (×108) | 2.04 ± 0.27 | 1.18 ± 0.10 | 1.53 ± 0.40 | 0.52 ± 0.20 |
| Spleen (×107) | 3.94 ± 0.68 | 3.70 ± 0.74 | 2.42 ± 0.71 | 2.25 ± 0.39 |
| Lymph nodes (×107) | 2.68 ± 0.50 | 2.70 ± 0.52 | 3.02 ± 0.87 | 3.15 ± 0.95 |