Table 2

Fitness in mice and in LB of FusR and compensated mutants.

StrainMutation (number of independent isolates found) Compensation selection conditionRelative fitness in miceRelative fitness in LB
EF-G*
JB124wtNA1.01.0
JB393P413LNANo growth0.41
JB2080wt (revertant) (2)LB1.01.0
JB2124P413L, G13C (3)LB0.941.0
JB2111P413L, L413Q (3)LB0.851.0
JB2105P413L, R407G (1)LB0.820.90
JB2117P413L, A378V (3)LB0.811.0
JB2115P413L, G13A (1)LB0.791.0
JB2108P413L, V363F (1)LB0.740.96
JB2119P413L, L413V (1)LB0.681.0
JB2104P413L, A66V (2)LB0.661.0
JB2112P413L, I294S (1)LB0.641.0
JB2122P413L, V376A (3)LB0.631.0
JB2114P413L, F444L (3)LB0.590.96
JB2109P413L, A378T (1)LB0.540.87
JB2113P413L, L387P (1)LB0.420.93
JB2120P413L, V291E (1)LB0.361.0
JB2110P413L, T423I (1)LB0.330.90
JB2153wt (revertant) (14)Mice1.01.0
JB2180P413L, F334L (7)Mice0.72ND
JB1777P413L, I294D (3)Mice0.521.0
JB1744P413L, P683L (1)Mice0.290.96
  • * Amino acid changes in EF-G (11).

  • Growth condition under which the compensated mutants were selected (7).

  • The relative fitness is defined as the generation time of the wild type divided by the generation time of the mutant (19). wt, wild type; NA, not applicable; ND, not determined.