Table 1

Nucleic acid aptamers for which three-dimensional structures have been determined. ND, not determined.

LigandNucleic acid*Affinity Kd [μM]3D structure
Theophylline RNA (4) ∼0.3NMR, 1EHT (5)
FMN RNA (6) ∼0.5 NMR, 1FMN (7)
AMPDNA (9) ∼6 NMR, 1AW4 (12)
RNA (8)∼10NMR, 1AM0, 1RAW (10, 11)
Arginine 2 DNA (15) ∼125NMR, 1OLD, 2ARG (18, 20)
RNA (16) ∼60 NMR, 1KOC (19)
Citrulline RNA (16) ∼65NMR, 1KOD (19)
Tobramycin 2 RNA (25) ∼ 0.009 NMR, 1TOB (32)
∼ 0.012 NMR, 2TOB (33)
Neomycin BRNA (26) ∼ 0.115 NMR, 1NEM (34)
HIV-1 Rev peptide 2 RNA (40) ∼ 0.004NMR, 1ULL, 484D (41, 42)
HTLV-1 Rex peptide RNA (43) ∼ 0.025NMR, 1C4J (44)
MS2 coat protein 3 RNA (45) NDX-ray, 5-7MSF (45, 46)
ThrombinDNA (47) ∼ 0.025 NMR, 148D (38); x-ray, 1HAO (39)
  • * The number of different sequences that have been studied is indicated.

  • The structure determination method (e.g., NMR, nuclear magnetic resonance) and the Protein Data Bank entry for the atomic coordinates are given.