Table 3

BAC and P1 clone-based sequencing. EDGP, EuropeanDrosophila Genome Project; BCM, Baylor College of Medicine; LBNL, Lawrence Berkeley National Laboratory (BCM and LBNL are the genomic sequencing centers of the BDGP).

Clone-based genomic sequencingGap closure: current status
Chromosomal regionGroupSizeFinished sequence (Mb)Draft sequence in joint assembly [BACs, (P1s)]Total sequenced BACs (P1s) in joint assemblyAdditional sequenced BACs in tiling pathPercentage of DNA sequence in contigs greater than
Clones Average coverage 30 kb 100 kb 1 Mb
X (1–3) EDGP 3 2.5  0  0 0 79.432.7 0
X (4–11) BCM 8.8 0.1*   0  1 72
X (12–20) LBNL 10 0 71 2.3× 71 10
2L LBNL 23 14.0103 (8) 1.6×  (5.3×) 119 (202) 2 97.8 91.416.9
2R LBNL 21.4 8.8 159 (32) 1.3×  (4.7×) 157 (186) 0 96.4 90.6 32.8
3L BCM24.4 0.1166 1.3× 170 50 95.177.7 0
3L LBNL 2.1  22 (7) 1.7×  (2.5×)  20 (32) 0
3R LBNL 28 2.1259 (9) 1.2×  (2×) 264 (27) 0 98.5 92.63.6
4 LBNL 1.2 0  16 1.4×  15 185.6 43.5 0
Total120 29.7 796 (56)817 (447) 135 93.7 77.5 9.9
  • * Sequenced at LBNL.

  • A tiling path of clones spanning 97% of the euchromatic portion of the genome was selected from the genome physical maps (10) for clone-based sequencing. The data include sequence that has been generated since the beginning of the publicly funded (BDGP and EDGP) genome sequencing projects. Tiling path clone identities were verified by screening the shotgun sequence for expected STS and BAC end-sequences, sequenced genes with known map locations from genes (and regions flanking P insertions), and sequences of neighboring tiling path clones. The average size of BAC clones in the tiling path is 163 kb. Sequencing methods are described in (66).