Table 1

Characteristics ofmaps and mags. Promoters with intracellular activation of fluorescence (Fig. 1A), trapped by differential fluorescence induction (15, 16), were sequenced with a primer derived from the pFPV27 vector (5) and their sequences compared with the National Center for Biotechnology Information (NCBI) sequence databases. Putative functions were assigned based on those determined or postulated for close homologs (>50% amino acid identity). Percent amino acid identities are derived from the mag sequences contained in the promoter insert spanning lengths of 64 to 130 amino acids. ND, not determined.

Promoter constructMagnitude of macrophage activation*Activity in granulomasM. tuberculosis homolog (% amino acid identity)Protein features or putative functionPossible role in mycobacterial persistence in vivo
map154ND
map24§33Yes PE-PGRS genes, Rv3812 and Rv1651c (Fig. 2C)Family of glycine-rich proteins unique to some pathogenic mycobacteria (17)Replication in host macrophages and persistence in granulomas (this study)
map253YesRv3416 (WhiB3) (96%)Transcriptional activator of early sporulation in Streptomyces coelicolor(27)Regulate sporulation-like features during long-term persistence
map 4932YesRv1200 (79%)Transmembrane (metabolite) transport protein in S. coelicolor, Escherichia coli, Hemophilus influenzae (17)Nutrient transport into vacuole of intracellular bacteria
map62|3Yes
map832NDRv0575c (77%)Oxidoreductase in S. coelicolor Counter oxygen radicals of macrophage
map 8532YesPE/PE-PGRS gene (53%)Mycobacterial glycine-rich protein family (17)
map86|6Yes
  • * map activity inside cells was determined as described in Fig. 1A.

  • map activity in granulomas of infected frogs was determined as described in Fig. 1C.

  • Genes described by Cole et al.(17).

  • § Although the beginning of mag24 is contained within the map 24 insert, its homology became apparent only after sequencing of the region downstream of the insert in the genomic clone.

  • | Inserts homologous toM. tuberculosis (map 86) or Mycobacterium leprae (map 62) genes but not adjacent to thegfp fusion.