Table 1

Activation of sAC by various salts.

Salt*% Basal
NaHSO3 138  ±  4.6
Na2SO3 164  ±  2.9
NaCI93  ±  11.8
Na2HPO4 75  ±  2.6
Na2SO4 66  ±  4.6
NaHCO3 411  ±  5.3
KHCO3 412  ±  6.1
  • * Salts were used at 50 mM.

  • AC activity shown relative to activity in the absence of any added salts. The assay included 40 mM MgCl2, 50 mM tris (pH = 7.5), and 10 mM [α32P]ATP (specific activity 6 × 103dpm/nmol). Values used were averages of triplicate determinations and are presented with SDs.