Table 1

SAGE analysis reveals previously characterized and novel pan endothelial markers. The most abundant characterized or novel tags derived by summing the tags from normal EC (N-ECs) and tumor EC (T-ECs) SAGE libraries are listed in descending order. For comparison, the corresponding number of SAGE tags found in HUVEC and HMVEC endothelial cell cultures, and several nonendothelial cell lines (14), are shown. Tag numbers for each group were normalized to 100,000 transcripts. A description of the gene product corresponding to each tag is given, followed by alternative names in parentheses. Some uncharacterized genes have predicted full-length coding sequence. The sequence CATG precedes all tags, and the 15th base (11th shown) was determined as described (38).

Genes determined by SAGE analysis to be potential pan endothelial markers
Tag sequenceN-ECsT-ECsHUVECHMVECCell linesDescription
Known genes
CATATCATTAA247501130872Angiomodulin (ANG, IGFBP-7, IGFBP-rP1, Mac25, TAF)*
TGCACTTCAAG328141000Hevin*
TTTGCACCTTT165841911154Connective tissue growth factor (CTGF, IGFBP-rP2)*
TTGCTGACTTT731312141Collagen, type VI, alpha 1*
ACCATTGGATT10267002Interferon induced transmembrane protein 1 (9-27, Leu 13)*
ACACTTCTTTC1044460622Guanine nucleotide binding protein 11
TTCTGCTCTTG7167118720Von Willebrand factor*
TCCCTGGCAGA66683133Cysteine-rich protein 2 (CRP-2, ESP-1, SmLIM)
TAATCCTCAAG2610634161Collagen, type XVIII, alpha 1*
ATGTCTTTTCT586517173Insulin-like growth factor-binding protein 4*
GGGATTAAAGC406730142CD146 (S-Endo 1, P1H12, Muc18, MCAM, Mel-CAM)*
TTAGTGTCGTA38699130SPARC (osteonectin, BM-40)*
TTCTCCCAAAT208616642Collagen, type IV, apha 2*
GTGCTAAGCGG24740102Collagen, type VI, alpha 2*
GTTTATGGATA355611111Matrix Gla protein (MGP)
Novel genes
CCCTTGTCCGA131104110PEM1 ESTs
CCCTTTCACAC5233000PEM2 ESTs
CAACAATAATA42251360PEM3 ESTs
GGCCCTACAGT2613230PEM4 ESTs/KIAA0821 protein
GCTAACCCCTG731010PEM5 ESTs
GGCACTCCTGT221319120PEM6 ESTs
TCACAGCCCCC2015850PEM7 ESTs
TTTCATCCACT2013020PEM8 ESTs, KIAA0362 protein
ATACTATAATT256200PEM9 ESTs
AATAGGGGAAA1319410PEM10 KIAA1075 protein
  • * Characterized genes that have previously been shown to be expressed predominantly in endothelium (10, 15–26).