SAGE tags elevated in tumor endothelium. The top 25 tags with the highest tumor EC (T-ECs) to normal EC (N-ECs) tag ratios are listed in descending order. To calculate tag ratios, we assigned a value of 0.5 in cases where zero tags were observed. The SAGE libraries are the same as those listed in Table 1. Tag numbers for each group were normalized to 100,000 transcripts. A description of the gene product corresponding to each tag is given, followed by alternative names in parentheses. TEM9 was uncharacterized at the outset of these studies but was recently characterized as a lectin present on ECs in culture and on microvascular vessels of human placenta in vivo (41).
| Tag sequence | Name | Acc.# | N-ECs | T-ECs | HUVEC | HMVEC | Cell lines | Description |
|---|---|---|---|---|---|---|---|---|
| GGGGCTGCCCA | TEM1 | AF279142 | 0 | 28 | 0 | 2 | 0 | ESTs, similarity to thrombomodulin |
| GATCTCCGTGT | TEM2 | AF279143 | 0 | 25 | 0 | 0 | 0 | ESTs, similarity to rat Rhes ras-related protein |
| CATTTTTATCT | TEM3 | AF070526 | 0 | 23 | 0 | 0 | 0 | ESTs |
| CTTTCTTTGAG | 0 | 22 | 6 | 20 | 1 | Regulated in glioma-like 7-1 (Dkk-3/REIC) | ||
| TATTAACTCTC | TEM4 | AB002335 | 0 | 21 | 1 | 3 | 1 | ESTs, similarity to JNK interacting protein-3a |
| CAGGAGACCCC | 0 | 16 | 2 | 0 | 0 | MMP-11 (stromelysin 3) | ||
| GGAAATGTCAA | 1 | 31 | 53 | 22 | 1 | MMP-2 (gelatinase A, 72-kD type IV collagenase)* | ||
| CCTGGTTCAGT | 0 | 15 | 0 | 0 | 0 | ESTs | ||
| TTTTTAAGAAC | TEM5 | AB040964 | 0 | 14 | 1 | 4 | 0 | ESTs |
| TTTGGTTTTCC | 5 | 139 | 0 | 16 | 0 | Collagen, type 1, alpha 2, transcript A* † | ||
| ATTTTGTATGA | 0 | 13 | 4 | 8 | 0 | Nidogen (enactin)* | ||
| ACTTTAGATGG | 1 | 23 | 0 | 15 | 0 | Collagen, type VI, alpha 3* | ||
| GAGTGAGACCC | 3 | 63 | 0 | 0 | 1 | Thy-1 cell surface antigen* | ||
| GTACACACACC | 0 | 10 | 0 | 0 | 0 | Cystatin S | ||
| CCACAGGGGAT | 2 | 38 | 0 | 2 | 1 | Collagen, type III, alpha 1 | ||
| TTAAAAGTCAC | TEM6 | AK001539 | 1 | 19 | 1 | 3 | 1 | ESTs |
| ACAGACTGTTA | TEM7 | AF279144 | 4 | 74 | 0 | 0 | 0 | ESTs, similarity with sea squirt nidogen |
| CCACTGCAACC | 1 | 18 | 0 | 1 | 0 | |||
| CTATAGGAGAC | TEM8 | AF279145 | 1 | 18 | 1 | 1 | 0 | ESTs, similarity with homeobox protein DLX-3 |
| GTTCCACAGAA | 0 | 9 | 0 | 3 | 0 | Collagen, type I, alpha 2, transcript B* † | ||
| TACCACCTCCC | 0 | 9 | 4 | 1 | 1 | ESTs | ||
| GCCCTTTCTCT | TEM9 | AB014609 | 1 | 17 | 3 | 1 | 2 | Endo180 lectin (macrophage mannose receptor family) |
| TTAAATAGCAC | 2 | 33 | 0 | 4 | 0 | Collagen, type 1, alpha 1* | ||
| AGACATACTGA | 1 | 16 | 1 | 0 | 0 | ESTs, DKFZP434G162 protein | ||
| TCCCCCAGGAG | 1 | 16 | 0 | 0 | 0 | Bone morphogenetic protein 1 (metalloprotease) |