Table 1

Classification of Arabidopsis ecotypes based on FRI alleles and flowering time. Flowering times (FT) of different ecotypes are from (10, 14, 15); ecotypes were classified as flowering early (E) or late (L), the latter being scored if the flowering time without vernalization was >75 days or the plants had more than 10 leaves. Where discrepancies occurred in the published data, flowering time was taken from our own unpublished observations. Stock center accession numbers of the ecotypes are included where available. The promoter difference was scored (+, present; –, deletion) using primers 5′-AGTACTCACAAGTCACAAC-3′ and 5′-GAAGATCATCGAATTGGC-3′. The presence of the Gly residue was scored by a CAPS marker (primers 5′-CCATAGACGAATTAGCTGC-3′ and 5′-AGACTCCAGTATAAGAAG-3′) and the presence of a Bsm F1 site. The 16-bp difference was scored (+, present; –, deletion) using primers UJ26 (5′-AGATTTGCTGGATTTGATAAGG-3′) and UJ34 (5′-ATATTTGATGTGCTCTCC-3′). Longitude and latitude coordinates are given for collection points of different ecotypes, except for Columbia whose geographical origin is unclear.

EcotypeAccession no.FTPromoterGly16 bpLongitudeLatitude
Group 1
Col933E+
Li-53178E+8°E50°N
En1136E+8°E50°N
Ws1638E+30°E52°N
Nd1390E+9°E53°N
MT-01380E+38°E56°N
Köln6003E+7°E51°N
OY-01436E+5°E61°N
Group 2
Ler 20E++15°E52°N
TSU-01564E++136°E34°N
RSCH-41494E++34°E56°N
Dijon910E++5°E47°N
GrE++16°E47°N
Group 3
H51L+++18°E59°N
Sf-21516L+++3°E42°N
Te1550L+++26°E63°N
Ko1288L+++12°E55°N
Can1064L+++15°W28°N
VimmerbyL+++16°E57°N
Lisse6033L+++5°E52°N
PU-2-3L+++15°E50°N
GOT-32L+++10°E52°N
LundL+++13°E56°N
Kent6009L+++1°E51°N
VästervikL+++16°E57°N
TAMM-46L+++23°E60°N
KZ-13L+++65°E48°N
PU-2-8L+++15°E50°N
MS-0905L+++38°E56°N
Group 4
EDI-01122L++3°W56°N
NC-6L++78°W35°N
DEM-4L++86°W40°N
AlgutsrumL++16°E57°N
DEM-2L++86°W40°N
BG-2L++123°W42°N
Group 5
Cvi1096E+++24°W16°N
Wil1594E+++25°E55°N
Shakhdara929E+++70°E39°N
KZ-9E+++65°E48°N
Kondara916E+++70°E39°N