Table 1

Quantitation of the number of donor cells in the forebrains of transplanted mice. A total of 21,682 cells was counted from seven animals. Ten to 20 random fields were photographed, and all DAPI-, NeuN-, or Y chromosome–positive nuclei were counted. Counts of cells represent an average from three independent investigators. The ratio of total cells to neurons was in good agreement with previous reports (45,46).

Age (months)DAPI-positive nuclei countedNEU-positive nuclei countedY chromosome– positive cellsY chromosome/NEU (double-labeled)Neu-positive nuclei in all cells (%)Y chromosomes in all cells (%)Y of Neu-positive cells (%)
4483119081206392.50.3
41322221605174.52.3
33675148313016403.51.1
34550182510515402.30.8
23731103916216284.31.5
11913464868244.51.7
11660380767234.61.8