Table 1

Analysis of CD8+ T cell responses in theMamu-A*01–positive monkeys by tetramer staining of freshly isolated PBMCs, tetramer staining of peptide-stimulated PBMCs, and functional chromium release cytotoxicity assays with peptide-stimulated PBMCs on day 63 after challenge (23, 30). Percent CD3+CD8+ cells that bind tetramer are shown for the tetramer assays. Percent-specific lysis at a 5:1 effector-to-target ratio are shown for the cytotoxicity assays.

MonkeyTetramer binding (fresh PBMCs)Tetramer binding (stimulated PBMCs)Cytotoxicity assay (stimulated PBMCs)
p11Cp41Ap68Ap11Cp41Ap68Ap11Cp41Ap68A
Controls
KPB0.200.1100300
KPE0.800.117011400
PKT0.10.10100000
TDE0.200210000
DNA vaccines alone
7021.50.102721135322
8117.90014101400
8201.300.118131602
DNA + IL-2/Ig protein
7122.90.10.1721144983
7724.40.30.1592818492611
7983.10.30.2633919432012
8392.20.30.14910439112
DNA + IL-2/Ig plasmid
4835.10.40.18874355554
7283.50.30.16316846292
8333.00.20.15922243192
8934.10.50.3867565544852