Table 1

The excised HMR cassette was not efficiently replicated. Cells were treated as described in Fig. 1. Noted are the final arrests in α factor (10 μg/ml) (G1) and the subsequent release and rearrest in benomyl (30 μg/ml) and nocodazole (10 μg/ml) (G2/M). The relative level of excised HMR was calculated as the ratio of the PhosphorImager units as follows: [(excised HMR/chromosome ΔHMR in either G1 or G2/M arrest)/(excised HMR/chromosome ΔHMR in G1 arrest)] × 100 ± σ ; n = 3. The ratios during the G1 arrest and the ratios during the G2/M arrest either in the presence of absence of Gal4-Sir1p were compared to determine whether they were similar, and data were analyzed by using the one-sided Wilcoxon rank sum test (P = 0.018). The ratio during the G2/M arrest in the presence of Gal4-Sir1p was compared to that in the absence of Gal4-Sir1p to determine whether they were similar, and data were analyzed by using the two-sided Wilcoxon rank sum test (P = 0.51). The ratio during the G1 arrest has been set to 100% to normalize the data between independent experiments, one of which is shown in Fig. 2B.

Expression of Gal4-Sir1pStage of arrestRelative level of excisedHMR (x 100 ± σ)
Gal4-Sir1pG1 100
Gal4-Sir1pG2/M62 ± 4.7
NoneG1 100
NoneG2/M50 ± 14