Table 1

Real-time qRT-PCR verification of selected colonization-associated changes in gene expression. Assays were performed with the pooled ileal RNA samples used for the microarray studies. Mean values for triplicate determinations ±1 SD are shown. See (7) for a comprehensive list of 71 genes that change with colonization.

Gene Fold Δ (relative to germ-free)
Na+/glucose cotransporter (SGLT-1) 2.6 ± 0.9
Colipase 6.6 ± 1.9
Liver fatty acid–binding protein (L-FABP) 4.4 ± 1.4
Metallothionein I (MT-I) −5.4 ± 0.7
Polymeric immunoglobulin receptor (pIgR) 2.6 ± 0.7
Decay-accelerating factor (DAF) 5.7 ± 1.5
Small proline-rich protein 2a (sprr2a) 205 ± 64
Glutathione S-transferase (GST)−2.1 ± 0.1
Multidrug resistance protein (Mdr1a)−3.8 ± 1.0
Lactase-phlorizin hydrolase −4.1 ± 0.6
Adenosine deaminase (ADA) 2.6 ± 0.5
Angiogenin-3 9.1 ± 1.8