Table 5

Mate-pair validation. Celera fragment sequences were mapped to the published sequence of chromosome 21. Each mate pair uniquely mapped was evaluated for correct orientation and placement (number of mate pairs tested). If the two mates had incorrect relative orientation or placement, they were considered invalid (number of invalid mate pairs).

Library typeLibrary no.Chromosome 21Genome
Mean insert size (bp)SD (bp)SD/mean (%)No. of mate pairs testedNo. of invalid mate pairs% invalidMean insert size (bp)SD (bp)SD/ mean (%)
2 kbp12,0811065.13,642 381.02,082904.3
21,9131527.928,0294131.51,9231186.1
32,1661758.14,405 571.32,1621587.3
10 kbp411,3858517.54,319 801.911,3706966.1
514,5231,87512.97,3551562.114,1421,4029.9
69,6351,03510.75,5731092.09,6069349.7
710,2239289.134,0793991.210,1907777.6
50 kbp864,8882,7474.216  16.365,5005,5048.4
953,4105,83410.991417018.653,3115,54610.4
1052,0347,31214.15,8715699.751,4986,58812.8
1152,2827,45414.32,6292138.152,2827,45414.3
1246,6167,37815.82,15321510.045,4189,06820.0
1355,78810,09918.12,24424911.153,06210,89320.5
1439,8945,01912.6199  73.536,8389,98827.1
BES1548,9319,81320.1144 106.947,8454,77410.0
1648,1304,2328.8195 147.247,9244,5819.6
17106,02727,77826.2330 164.8152,00026,60017.5
18160,57554,97334.2155  85.2161,75027,00016.7
19164,15519,45311.9642 446.9176,50019,50011.05
Sum102,8942,7682.7
(mean = 2.7)