Table 1

Genetic interactions between lip-1 and genes controlling vulval induction.

RowGenotype*Average inductionNumber
1Wild-type3.0Many
2 lip-1(lf) 3.028
3 let-23(rf) 1.149
4 let-23(rf); lip-1(lf) 2.447
5 lin-7(lf) 0.326
6 lin-7(lf); lip-1(lf) 2.543
7 sem-5(rf) 1.148
8 lip-1(lf); sem-5(rf) 2.940
9 mpk-1(rf) 1.044
10 mpk-1(rf); lip-1(lf) 2.856
11 [HS-mpk-1(+)] 3.040
12 [HS-mpk-1(+)]; lip-1(lf) 5.922
13 lin-25(rf) 1.532
14 lip-1(lf); lin-25(rf) 2.146
15 [lin-31-lip-1] 0.840
16 [lip-1::gfp] 2.856
17 [lip-1::nls::gfp] 0.937
18 let-60(gf) 4.345
19 let-60(gf); [lip-1::nls::gfp] 3.023
20 [HS-mpk-1(+)] 5.641
21 [HS-mpk-1(+)]; [lip-1::nls::gfp] 3.051
22 lin-1(rf) 5.933
23 lin-1(rf); [lip-1::nls::gfp] 5.838
  • * Alleles used: lip-1(zh15) (this study);let-23(sy1), lin-7(e1413),sem-5(n2019), mpk-1(oz140),gaIs36[HS-mpk-1(+), EF1α-D-mek(+),unc-30(+)] (rows 11 and 12), lin-25(n545),zhEx22[lin-31-lip-1],zhEx16[lip-1::gfp], andzhEx17[lip-1::nls::gfp] (all this study); let-60(n1046gf),gaIs37[HS-mpk-1(+), EF1α-D-mek(+),unc-30(+)] (rows 20 and 21), andlin-1(n304).

  • Average induction indicates the average number of VPCs adopting induced (1° or 2°) fates per animal.

  • The phenotype of these strains was scored at 25°C.