Table 1

Tissue tropism of H5N1 transfectant viruses. BALB/c mice, anesthetized with methoxyflurane, were infected intranasally with 50 μl of virus (100 PFU). Three mice from each infected group were killed on day 3 or day 6 after infection (or both) for virus titration. When virus was not recovered from all three mice, individual titers were recorded. (–) Virus not isolated.

VirusDayVirus titer [(mean log PFU ± SD)/g] in
LungsNasal turbinatesSpleenHeartKidneysBrainPancreasColon
A/Hong Kong/483/9736.7 ± 0.14.0, 4.33.3 ± 0.4
66.3 ± 0.35.7 ± 0.21.74.0 ± 0.21.73.8 ±  1.02.9 ± 0.63.2 ± 0.2
A/Hong Kong/486/9734.0 ± 0.1
65.5 ± 0.5
HK483RG37.1 ± 0.23.4 ± 0.64.1 ± 0.22.2 ± 0.31.71.4, 1.4
66.1 ± 0.15.4 ± 1.34.8 ± 0.32.9 ± 0.73.9 ± 1.02.3 ± 0.53.7 ± 1.0
HK486RG(HA-227S)35.3 ± 0.3
66.5 ± 0.25.3
HK486RG(HA-227I)33.8 ± 0.7
65.3 ±  0.3
HK3/6PB266.7 ± 0.73.8 ± 0.74.5, 3.1
HK3/6HA227I66.5 ± 0.2
HK3/6HA227S67.6 ± 0.16.8 ± 1.02.35.6 ± 0.82.7 ± 0.55.6 ± 0.43.94.5 ± 0.4
HK483/6NA66.4 ± 0.12.7 ± 0.73.4 ± 0.12.1 ± 0.22.4 ± 0.52.52.5 ± 0.2
HK6HA227S/3PB24* 8.3 ± 0.27.1 ± 0.43.6 ± 0.15.9 ± 0.26.1 ± 0.36.5 ± 0.24.7 ± 0.35.4 ± 0.2
HK6HA227I/3PB266.1, 6.73.2
HK6HA227S/3NA67.3 ± 0.12.1, 3.8
HK6HA227I/3NA65.1 ± 0.9
HK6PB2–627K5* 7.7 ± 0.16.6 ± 0.85.4 ± 0.44.2 ± 0.66.6 ± 0.22.6, 5.15.4 ± 0.9
HK3HAavir36.4 ± 0.11.5, 2.2
6
  • * Mice were killed on days 4 and 5 because all mice died on days 5 and 6 in the preliminary experiments.