Table 1

Types of embryo sacs attracting pollen tubes after laser cell ablation. Complete embryo sacs were operated on by laser at the start of cultivation and were monitored until 24 hours later to determine whether pollen tubes were attracted. The number of embryo sacs observed was accumulated from many independent experiments.

Type of embryo sacExistence of each cellFrequency of attraction (%)
ECCCSYSY
Complete++++48/49 (98%)
Complete, with killed ovular cells++++30/30 (100%)
One cell killed+++35/37 (94%)
+++10/10 (100%)
+++35/49 (71%)
Two cells killed++13/14 (93%)
++11/18 (61%)
++10/14 (71%)
++0/77 (0%)
Three cells killed+5/8 (63%)
+0/20 (0%)
+0/18 (0%)
All four cells killed0/79 (0%)