Table 1

Cell biological responses and effects of inhibiting the α5 integrin (16–18). RS, relative stimulation versus fibronectin; α5I, α5 inhibition expressed as a percentage relative to the untreated control on the same substrate. Assay data are expressed as follows. Attachment assay: Arbitrary units (a.u.) denote relative numbers of attached cells compared to fibronectin 10 min after plating. Cell morphologyassay: EFF = elliptical form factor (length/breadth) at 5 or 18 hours. Movement assay: mean velocity determined by time-lapse recording. Proliferation assay: % BrdU = [BrdU-positive cells (proliferative)]/[lamin B–positive cells (total)] × 100. *P < 0.05, **P < 0.01, ***P < 0.001; statistical analyses were by analysis of variance or by Kruskal-Wallis tests for the attachment assays.

AssayFibronectin3D matrix2D matrix2D mixLamininCollagen ICollagen gel
RSα5I (%)RSα5I (%)RSα5I (%)RSα5I (%)RSα5I (%)RSα5I (%)RSα5I (%)
Attachment (a.u.)1.036*** 6.3*** 88*** 0.6*** 131.1 62*** 0.5*** 30.970.5*** −2
Cell morphology
(EFF, 5 hours)1.029* 2.9*** 68*** 0.9131.0 320.920.8−170.9−9
(EFF, 18 hours)1.034* 1.9*** 56*** 1.9*** 71.6** 81.131.3181.2−11
Movement (μm/hour)1.034* 1.5*** 65*** 1.7** 121.9*** 21.2−111.1−30.9−4
Proliferation1.027* 2.2*** 78*** 0.6* 20.8−150.6* −180.7−30.7* 20
(% BrdU positive)