Table 1

Requirement of γ-secretase for HRG-dependent growth inhibition. T47D cells were plated in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal bovine serum. After overnight incubation, the cell number was determined (748,000 cells per well) and the medium was changed to serum-free DMEM. Additions of HRG (20 ng/ml), EGF (25 ng/ml), or compound E (10 nM) were made as indicated. After 2 days, the medium with fresh additions was changed; final cell numbers were determined after 4 days. Changes relative to no additions (100%) are also shown.

AdditionsFinal cell number (×103)Relative change (%)
None1007 ± 65100
HRG373 ± 3937
HRG + compound E978 ± 9497
EGF1418 ± 188141
EGF + compound E1664 ± 192165
Compound E863 ± 13486