Table 1

Inhibition of constitutive Hsp70 activity enhanced α-synuclein–induced loss of dopaminergic neurons in the DM clusters. The UAS-Hsc4.K71S transgene encodes a dominant-negative form of the constitutive Drosophila Hsp70 protein, Hsc4 (22). Dopaminergic neurons were visualized by immunostaining for tyrosine hydroxylase. Serial sections through three to five fly heads were examined for each time point.

ConditionMean number of dopaminergic neurons ± SEM
1 day20 days
Control16.5  ±  2.516.3  ±  0.3
(w;Ddc-GAL4/+)
Synuclein 15.5  ±  3.58.0  ±  0.6
(w;Ddc-GAL4/+;UAS-α-syn/+)
Hsc4.K71S alone 19.5  ±  0.59.7  ±  1.8
(w;Ddc-GAL4,UAS-Hsc4.K71S/+)
Synuclein and Hsc4.K71S 6.7  ±  2.36.3  ±  1.7
(w; Ddc-GAL4, UAS-Hsc4.K71S/+;UAS-α-syn/+)