Table 1

Methods used to determine the size of lipid rafts give different results. The conventional fluorescence resonance energy transfer (FRET) results could mean that there are no rafts (0 size) or that the density of the marker protein is too low to support intermolecular FRET (65). 5′NT, 5′ nucleotidase; FR, folate receptor; DAF, decay accelerating factor; PALP, placental alkaline phosphatase; HA, hemagglutinin; DMPE, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine.

MethodCellMarkerSize (nm)No. of moleculesRef.
ConventionalMDCKGM100(37,65)
FRETHeLa5′NT
NRKFR
FAOCD59
Molecular cross-linkingBHKDAF>4 nm15(66)
Laser trapBHKPALP ∼50 nm3000(67)
HA
DepolarizationCHOFR<70 nm6000(68)
FRET
Single-particle trackingC3HGM1 ∼200 nm50,000(69)
Thy-1
Single dye tracingHASM cellsDMPE∼700 nm600,000(70)