Table 2

Hyp phenotype of mutants in daf-2 life-span pathway. All animals were raised at 20°C. Adults 2 days post L4 were exposed to <0.3% oxygen at 28°C for 20 hours then scored after a 24-hour recovery period. The genotypeage-1(mg44)m+z− is zygotic homozygousmg44 from mg44/+ mothers.

GenotypeMutationPercent deadAnimals (n)
+/+wild type95.5 ± 1.22568
age-1(hx546) rf78.8 ± 10.3 254
age-1(mg44)m+ z– null46.2 ± 3.6* 47
akt-1(mg144) gf79.3 ± 9.3 489
pdk-1(mg142) gf98.4 ± 1.0 518
pdk-1(sa680) rf42 ± 4.4* 56
daf-2(e1370) rf3.6 ± 1.3* 835
age-1(mg44);akt-1(mg144) null;gf7.5 ± 3.5* 343
daf-2(e1370);akt-1(mg144) rf;gf77.5 ± 3.8 461
age-1(mg44);pdk-1(mg142) null;gf12.5 ± 5.1* 83
daf-2(e1370);pdk-1(mg142) rf;gf44.4 ± 5.6* 39
daf-18(nr2037) null100 ± 0 127
daf-2(e1370);daf-18(nr2037) rf;null97.2 ± 0.9 415
daf-2(e1370);daf-18(e1375) rf;rf27.6 ± 13.3* 57
daf-16(mgDf50) null100 ± 0 169
daf-16(mgDf47);daf-2(e1370) null;rf100 ± 0 100
daf-16(mg54);daf-2(e1370) null;rf96.0 ± 0.8 167
daf-16(m26);daf-2(e1370) null;rf86.8 ± 5.3 189
old-1(mk1) null79.8 ± 11.3 560
old-1(mk1);daf-2(e1370) null;rf3.0 ± 0.6* 161
  • P < 0.01 by Fisher's exact test:

  • * Versus wild type;

  • Versus e1370.