Table 1

Characterization of whi mutants. Strains at or below the growth rate–cell size baseline as defined in Fig. 2B are listed in order of increasing size; ± 1 SD is reported for each parameter. All strains listed here showed tight linkage between kanR-marked gene deletion and the Whi phenotype. Homologs have 25% amino acid identity over more than half the protein, except for SPAC22G7.02 and importin 13, which only have ∼20% identity. R, resistance;S, sensitivity to mating pheromone α-factor, relative to the response of the wild-type strain (WT) (26). ORF overlaps between YGR064W and SPT4and between YNL227C, YNL226W, andYNL228W preclude definitive assignment of the Whi phenotype. NA, not applicable.

Deleted geneDoubling time (min)Haploid cell size (fl)1N DNA content (%)% buddedResist.Heterozygote cell size (fl)Known roleHomolog(s)
DaughterMedianDaughterMedianS. pombeHuman
SFP1 220 ± 178 ± 125 ± 173 ± 024 ± 4 S 46 ± 276 ± 1UnknownSPAC16.05c
SCH9 153 ± 29 ± 128 ± 164 ± 343 ± 2 S 40 ± 469 ± 2Growth controlSck1/2Akt1/2/3 (PKB)
PRS3 135 ± 317 ± 130 ± 144 ± 165 ± 2 S 47 ± 279 ± 4Nucleotide metabolismSPCC1620.06*Prps1/2*
WHI3 108 ± 317 ± 031 ± 142 ± 257 ± 7 R 48 ± 177 ± 4LocalizesCLN3mRNA
WHI5 88 ± 218 ± 032 ± 1 34 ± 4 68 ± 7 R 52 ± 290 ± 3 Unknown
RPA49 137 ± 816 ± 1 33 ± 1 57 ± 6 44 ± 7 S 47 ± 3 78 ± 2 rRNA transcriptionRpa49FLJ13390
YNL227C 124 ± 916 ± 1 34 ± 1 50 ± 2 42 ± 5 WT 45 ± 0 73 ± 2UnknownSPAC6B1208
YNL226W 126 ± 1116 ± 1 34 ± 1 52 ± 4 45 ± 4 WT 45 ± 0 74 ± 2Unknown
PHO5 125 ± 3 17 ± 034 ± 1 46 ± 0 49 ± 1 WT 43 ± 1 71 ± 1 Acid phosphatasePho1
HXK2 86 ± 4 19 ± 034 ± 1 51 ± 6 59 ± 3 WT 49 ± 3 81 ± 3 Glucose signalingHxk1* Hk1*
YHR034C 111 ± 718 ± 0 35 ± 0 61 ± 1 38 ± 6 WT 50 ± 4 84 ± 3UnknownFLJ20643
SKY1 97 ± 418 ± 0 35 ± 1 52 ± 1 47 ± 7 WT 49 ± 2 80 ± 3 SR protein kinaseDsk1Srpk1
KAP122 91 ± 119 ± 1 35 ± 1 52 ± 4 53 ± 6 WT 51 ± 5 84 ± 4Nuclear transportSPAC22G7.02importin 13
SSF1 107 ± 818 ± 0 36 ± 153 ± 1 41 ± 6 WT 52 ± 182 ± 4 Nucleolar proteinSPAC6B12.01Ppan
RPA14 97 ± 219 ± 036 ± 1 48 ± 2 61 ± 5 WT 53 ± 6 85 ± 8 rRNA transcription
YGR111W 103 ± 6 19 ± 036 ± 1 46 ± 1 56 ± 2 WT 51 ± 2 84 ± 5Unknown
YCR061W 91 ± 119 ± 136 ± 1 45 ± 2 57 ± 10 WT 45 ± 2 77 ± 1Unknown
PTK2 88 ± 3 20 ± 136 ± 1 46 ± 1 45 ± 2 WT 51 ± 0 83 ± 0 Protein kinase
TOM1 99 ± 0 18 ± 0 37 ± 144 ± 2 57 ± 2 WT 45 ± 171 ± 2 Ubiquitin-protein ligaseSPAC19D5.04
GPA2 91 ± 620 ± 0 37 ± 0 55 ± 7 53 ± 12 WT 50 ± 4 82 ± 5 cAMP signalingGpa2* Gnai1*
KEL1 87 ± 421 ± 0 37 ± 1 42 ± 1 62 ± 7 WT 52 ± 1 82 ± 3 Cell polarityTea1
SWE1 91 ± 2 19 ± 038 ± 1 49 ± 1 50 ± 1 WT 50 ± 4 83 ± 3 Inhibitory Cdc28 kinaseWee1Wee1
YGR064W 91 ±1 21 ± 139 ± 1 41 ± 2 69 ± 1 WT 53 ± 2 84 ± 2Unknown
CDH1 115 ± 4 14 ± 240 ± 1 35 ± 3 64 ± 6 S 51 ± 589 ± 7 Cyclin degradationSrw1hCdh1
WHI6 102 ± 519 ± 043 ± 350 ± 252 ± 3 R 50 ± 384 ± 1Unknown
WT87 ± 6 23 ± 0 42 ± 244 ± 1 59 ± 5 WT 50 ± 282 ± 0 NANANA
  • * Proteins that belong to members of highly conserved families with multiple S. pombe and/or human homologs.

  • Strains subjected to epistasis analysis.