Table 1

Classes and size of replication intermediates in the presence of hydroxyurea (HU). Data related to Figs. 1 and 2. Total number of analyzed RIs is in parentheses. wt, wild type.

Cells and treatmentBubbles (%)Y-shaped molecules + broken bubbles (%)RI (n)Size of replicating bubbles (bp)RI (n)
wt + HU 0.5 hours71.328.7(230)3025  ±  1732(149)
wt + HU 1 hours74.525.5 (141)4227  ±  1807(100)
wt + HU 2 hours7624(104)7484  ±  4155(76)
rad53 + HU 0.5 hours70.829.2 (181)2099  ±  1681(106)
rad53 + HU 1 hours77.522.5 (165)2747  ±  1355(87)
rad53 + HU 2 hours45.854.2 (144)3116  ±  1991(56)