Table 2

Comparative response of human polyclonal T cell lines (coded by numbers) derived from intestinal biopsies of Celiac Sprue patients to LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF (33-mer) and representative gliadin epitopes defined by earlier studies. Each antigen was pretreated with tTGase to elicit an enhanced T cell response. Peptide 1206 (YQQLPQPQQPQQSF- PQQQRPF; corresponding to γ-I); Peptide 1258 (PQPQLPYPQPQLPY; corresponding to α-II); Peptide 1306 (IIQPQQPAQ; corresponding to γ-II); Peptide 1317 (LQPQQPFPQQPQQPYPQQPQ; corresponding to γ-III + γ-V). Blank cell implies no response was observed for these peptides against the corresponding T cell lines at deamidated peptide concentrations up to 10 μM.

EC50(μM)
411.1412.1432.1.4450.2.2422.02.4.2488.3.1437.1.1425.1419.1435.6467.2E.1410.1380.1421.1.4
Peptide 12062.5
Peptide 12581.62.50.620.620.161.62.540.160.62.536
peptide 13060.652.5
Peptide 13172.52.51.653
33-mer0.10.150.050.040.050.080.080.40.0150.040.10.40.60.6