Table 1

NAD+-, CobB-dependent activation of acetyl-CoA synthetase.

Overexpression host genotype*
cobB+cobB
AdditionAcs specific activityAdditionAcs specific activity
None 119  ±  5None Not detectable
BSA§ 351  ±  14 BSA9 ± 0.1
CobB 806  ±  9CobB22 ± 4
CobB, BSA810  ±  4 CobB BSA19 ± 4
CobB, NAD 809  ±  8 CobB, NAD 480 ± 4
BSA, CobB, NAD 902  ±  10 BSA, CobB, NAD 483 ± 19
  • * Relevant genotype of the strain from which the Acs protein was purified.

  • All reaction mixtures contained coenzyme A, Mg(II)/ATP, and Acs proteins.

  • Specific activity is defined as nanomoles of acetyl CoA formed per minute per milligram. Results are the average of three independent determinations.

  • § BSA, bovine serum albumin.