Table 1

Pumping rates of larvae and adults treated with respiratory chain RNAi.

Feeding rate (pumps per minute)*
RNAi treatmentDay 2Day 3Day 4Day 5
Vector118  ±  15 (0/8)140  ±  21 (0/8)145  ±  24 (0/8)131  ±  49 (1/10)
Complex III (cyc-1) 97  ±  21 83  ±  12 84  ±  1275  ±  12
larval + adult(0/10)(0/10)(0/10)(0/10)
Complex III (cyc-1) 79  ±  62 112  ±  80 80  ±  5969  ±  45
adult only(5/12)(3/12)(4/12)(4/12)
Complex V (atp-3) 98  ± 15 87  ±  10 80  ±  975  ±  8
larval and adult(0/10)(0/10)(0/10)(0/10)
Complex V (atp-3) 88  ±  80 98  ±  64 83  ±  5989  ±  54
adult only(6/14)(5/14)(4/14)(4/14)
  • * Mean ± SD. The number of pharyngeal pumps observed in an adult animal in 1 min at 25°C. Number in parentheses is the number of animals that produced fewer than 50 pumps per minute/total number of animals tested.