Table 1

Polarization of GFP-Cdc42 in wild-type and mutant strains.

Strains and conditions*Cells with 1 cap (%)Cells with 2 caps (%)
wt, 23°C 53.3 ± 3.434.0 ± 3.3
wt, 30°C 71.2 ± 1.06.6 ± 2.5
wt, shift to 30°C§ 52.0 ± 1.432.2 ± 2.9
Δbud1, 30°C 55.0 ± 0.88.3 ± 2.6
wt, 30°C, Noc| 73.6 ± 2.43.0 ± 0.8
myo2-66, 23°C62.0 ± 12.04.7 ± 2.9
myo2-66, shift to 30°C2.0 ± 1.60
myo2-66, 30°C00
sec3-2, 23°C55.0 ± 6.20.3 ± 0.5
sec3-2, shift to 30°C§9.3 ± 2.50
sec3-2, 30°C00
Δtpm2, tpm1-2, 23°C 58.6 ± 5.30
Δtpm2, tpm1-2, shift to 30°C§ 2.7 ± 1.70
Δtpm2, tpm1-2, 30°C 4.0 ± 2.20
sec6-4, 23°C70.3 ± 4.21.0 ± 0.8
sec6-4, shift to 30°C§11.0 ± 3.30
sec6-4, 30°C7.0 ± 1.60
  • * All strains are in the Δcln1,2,3, MET3-CLN2, GAL-MG-Cdc42Q61L background, which is designated as wild type (wt).

  • Cells were arrested for 4 hours and then induced for 5 hours.

  • Cells were arrested for 3 hours and then induced for 4 hours.

  • § After 4 hours of induction at 23°C cells were shifted to 30°C for 30 min.

  • | Cells were treated with 10 μM nocodazole during induction of MG-Cdc42Q61L.