Table 2

Spontaneous and GC-dependent spectra for K. lactis URA3. For 1-bp deletions, 1-bp insertions, BPSs, and complex mutations, numbers in parentheses indicate percentage of total mutations represented by each respective mutation. –1 deletions and +1 insertions are divided into events occurring in either homonucleotide runs ≥2 nucelotides (nt) or nonrepeating sequences. spont., spontaneous; comb., combined data from asynchronous and G1 cells.

Type of mutationWT
spont.
Sc-URA3
spont. (9)
WT GC
comb.
pol2-4
GC
pol2-4
spont.
pol3-01
GC
pol3-01
spont.
bp substitutions46 (75%)167 (81%)56 (54%)21 (46%)41 (82%)48 (98%)49 (98%)
      Transitions1946321082017
      Tranversions271212411332832
–1 deletions5 (8.0%)22 (11%)32 (31%)12 (26%)001 (2.0%)
      –1 in homo nt3142912000
      –1 in mono nt2830001
+1 insertions1 (2.0%)3 (1.0%)1 (1.0%)7 (15%)9 (18%)1 (2.0%)0
      +1 in homo nt1217910
      +1 in mono nt0100000
Template switches0n/a12 (12%)5 (11%)000
      Ectopic (Sc-ura3-52)0n/a22000
      Quasipalindrome0n/a103000
Other complex9 (15%)15 (7.0%)2 (2.0%)1 (2.0%)000
Total mutations6120710446504950