Table 1.

Data collection and refinement statistics.

β2AR-T4L
Data collection (APS GM/CA CAT 23ID-B, 10-μm beam) *
Space group C2
Cell dimensions
    a, b, c (Å) 106.3, 169.2, 40.2
    β (°) 105.62
Number of reflections processed 245,571
Number of unique reflections 26,574
Resolution (Å) 50 to 2.4 (2.5 to 2.4)
R sym 12.7 (67.8)
Mean I/σ(I) 9.6 (2.2)
Completeness (%) 99.5 (99.1)
Redundancy 9.4 (4.8)
Refinement *
Resolution (Å) 20 to 2.4 (2.46 to 2.4)
Number of reflections (test set) 25,247 (1310)
Rwork / Rfree 19.8 (27.0) / 23.2 (30.1)
Number of atoms 3805
    Protein 3544
    Ions, lipids, ligand, and other 213
    Water 48
Overall B values (Å2) 82
    β2AR 77
    T4 lysozyme 75
    Carazolol 55
    Lipid 100
RMSD
    Bond lengths (Å) 0.013
    Bond angles (°) 1.5
Ramachandran plot statistics (%) (excluding Gly, Pro):
    Most favored regions 94.8
    Additionally allowed regions 5.0
    Generously allowed regions 0.2
    Disallowed regions 0
  • * Highest-resolution shell is shown in parentheses.

  • Rsym = Σhkl |I(hkl) - 〈I(hkl)〉|/Σhkl(hkl), where 〈I(hkl)〉 is the mean of the symmetry-equivalent reflections of I(hkl).