Table 1.

Chemical and SSU rRNA tag characteristics of the two sites.

FS312FS396
Vent name Bag City Marker 52
Sample year 2003 2004
Volume filtered (ml) 1003 2000
Cells ml-1 (range) 1.21 × 105 (9.77 × 104 to 1.26 × 105) 1.57 × 105 (1.02 × 105 to 2.12 × 105)
Culturable* hyper/thermophilic heterotrophs per liter 140 to 4200 20 to 720
DNA recovered (μg) 0.9 2.4
Total number of archaeal V6 tag sequences 200,199 16,428
Total number of bacterial V6 tag sequences 442,058 247,662
Total number of ϵ-proteobacterial V6 tag sequences 122,823 147,515
Depth (m) 1,537 1,529
Latitude and longitude 45.92°N, 129.99°W 45.94°N, 129.99°W
Average temperature (°C) 31.2 24.4
Maximum temperature (°C) 31.4 24.9
H2S/ΔT (μmol kg-1 °C-1) 7.2 18.9
pH 6.26 5.08
Mg (mmol/kg) 48.3 50.8
Alkalinity (meq/liter) 2.4 3.7
Mn (μmol/kg) 19.8 4.8
Fe (μmol/kg) 0.8 7.9
Silica (mmol/kg) 1.46 1.07
  • * Cultured at 70° or 90°C in 0.3% yeast extract and peptone with elemental sulfur; Ar headspace.

  • Trimmed reads that passed quality control [as described in (11)].