Table 1.

Poliovirus specific infectivity and attenuation. PLD50 is the amount of virus that caused paralysis in 50% of infected mice.

VirusAbsorbance at 260 nmPurified particles/ml * Purified PFUs/mlRelative specific infectivity PLD50 (particles) PLD50 (PFUs)
PV(M)-wt 0.956 8.97 × 1012 6.0 × 1010 1.00 104.0 101.9
PV-Max 0.842 7.91 × 1012 6.0 × 1010 1.04 104.0 101.9
PV-MinXY 0.944 8.87 × 1012 9.6 × 108 0.015 107.1 103.2
PV-MinZ 0.731 6.87 × 1012 5.1 × 108 0.010 107.3 103.2
  • * Calculated by the formula 9.4 × 1012 particles/ml = 1 OD260 (5).

  • Calculated by dividing the PFU/ml of purified virus on HeLa R19 cells by particles/ml, and then normalizing to the wild-type value. In absolute terms, wild-type specific infectivity was 1/137.

  • Calculated after intracerebral injection of virus into CD155 tg mice at varying doses.