Table 2.

pen2 and pcs1 mutants exhibit feedback inhibition at the level of MYB51 expression. Ratio of mRNA abundance in mutant and/or INA-pretreated relative to water-pretreated wild-type plants after Flg22 treatment as determined by qRT-PCR. HAT, hours after treatment with Flg22.

Genotype/chemicalHATPAMP-inducible gene
PEN2 PCS1 PEN3 CYP81F2 ERF1 MYB51 ASA1 CYP79B2 CYP79B3 CYP83B1 SUR1 UGT74B1 AtST5a
pen2-1 3 0.17* 0.77 0.89 1.16 1.06 0.48* 0.25* 0.30 0.10* 0.20* 0.39* 0.21* 0.17*
6 0.13* 1.10 0.60 0.52* 0.42 0.40* 0.19* 0.15* 0.34* 0.28* 0.37* 0.19* 0.24*
pen2-1/INA 3 0.19* 0.55 0.98 0.96 1.05 0.52* 0.51* 0.46* 0.09* 0.31* 0.60* 0.29* 0.28*
6 0.22* 1.45 0.54* 0.74* 0.51* 0.59* 0.40* 0.43* 0.38* 0.35* 0.66 0.33* 0.41*
pcs1-2 3 1.12 0.08* 0.89 1.49* 0.81 0.67* 0.32* 0.19* 0.29* 0.32* 0.48* 0.30* 0.24*
6 1.00 0.22* 0.94 1.21 0.63 0.80 0.49* 0.41* 0.17* 0.50 0.64* 0.54* 0.51*
pcs1-2/INA 3 0.89 0.12* 0.98 1.97 1.26 0.77* 0.76* 1.04* 0.27* 0.46* 0.79* 0.43* 0.46*
6 0.86 0.25* 0.91 1.08 1.10 0.88 0.79 0.49* 0.23* 0.67 0.89 0.62 0.56*
WT/INA 3 0.78 0.86 0.98 1.57* 0.90 1.85* 2.69* 6.12* 1.29 1.80* 2.04* 2.05* 1.81*
6 0.72 1.31 0.83 0.99 0.88 1.17 1.33 1.64 1.69 1.14 1.14 1.00 0.97
pen3-1 3 1.19 1.04 1.09 0.82 1.40 0.40* 0.32* 0.14* 0.19* 0.30* 0.42* 0.26* 0.24*
6 1.20 1.46 1.15 1.10 1.21 0.85 0.46 0.53 0.39 1.30 0.86 0.97 0.96
  • * P < 0.01, two-tailed t test; false discovery rate < 0.05.

  • Pairwise comparisons to water-pretreated wild-type plants.

  • Pairwise comparisons to INA-pretreated wild-type plants.