Table 1

bNOS protection against a wide spectrum of antimicrobials. A representative list of chemicals from the Phenotype MicroArray screen that preferentially inhibit the growth of nos-deficient B. subtilis. Negative numbers indicate the relative growth inhibition (as provided by Biolog, fig. S1) of the Δnos strain compared to that of the wt strain.

Δnos inhibitionChemicalProposed biological effect/chemical type
–765-Chloro-7-iodo-8-hydroxyquinolineDNA intercalator/quinolone
–628-HydroxyquinolineDNA intercalator/quinolone
–678-Hydroxy-5-nitroquinolineDNA intercalator/quinolone
–80NovobiocinDNA intercalator/quinolone
–209AcriflavineDNA intercalator/acridine
–449-AminoacridineDNA intercalator/acridine
–109ProchlorperazineDNA intercalator/phenothiazine
–77ChlorpromazineDNA intercalator/phenothiazine
–45ProchlorperazineDNA intercalator/phenothiazine
–68PenimepicyclineProtein synthesis/tetracycline
–64SisomicinProtein synthesis/aminoglycoside
–40GentamicinProtein synthesis/aminoglycoside
–252CephaloridineCell wall metabolism/cephalosporin
–1247-Aminocephalosporanic acidCell wall metabolism/cephalosporin
–110CefotaximeCell wall metabolism/cephalosporin
–173CefuroximeCell wall metabolism/cephalosporin
–114AmpicillinCell wall metabolism/lactam
–79MoxalactamCell wall metabolism/lactam
–786-Aminopenicillanic acidCell wall metabolism/lactam
–73AmoxicillinCell wall metabolism/lactam
–39AzlocillinCell wall metabolism/lactam