Table 2 Reduced genomes resulting from the DBT cycles, ultimately leading to syn3.0.

Column 1 indicates the round of genome design (dashes indicate the starting genome, syn1.0), column 2 gives the size of the designed genome (in kilobase pairs), and column 3 gives the number of mycoplasma genes in the design. Column 4 shows the genome composition for key viable cell strains; for nonviable designs, a viable strain with the highest number of segments from the design is shown, as well as a more robust alternative for RGD1.0 (fourth row) and a smaller derivative for RGD2.0 (sixth row, syn2.0). Column 5 gives the size of the genome corresponding to column 3, and column 6 shows a quantitative or qualitative estimate of the growth rate of cells with the genome described in column 4.

1. Genome
design
2. Design
size
3. Number
of design
genes
4. Cellular genome
segment composition
for key viable strains
5. Cellular
genome size
6. Growth
rate
-901syn1.0: all eight syn1.0 segments1079 kbpDoubling time, td = 60 min
HMG483 kbp460HMG segment 2 + 7/8 syn1.01003 kbpSlow-growing
RGD1.0544 kbp483RGD1.0 segments 2,6,7,8 + syn1.0 segments 1,3,4,5758 kbpSlow-growing
" "RGD1.0 segments 1,2,4,6,8 + syn1.0 segments 3,5,7718 kbpSlow-growing
RGD2.0575 kbp512RGD2.0 segments 1,2,3,4,6,7,8 + syn1.0 segment 5617 kbp?
""syn2.0: RGD2.0 segments 1,2,3,4,6,7,8 + syn1.0 segment
5 with genes MMSYN1_0454 to -0474 and MMSYN1_0483 to -0492 deleted
576 kbptd = 92 min
RGD3.0531 kbp473syn3.0: all eight segments of RGD3.0531 kbptd = 180 min